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KMID : 0903619860270040314
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Journal of the Korean Society for Horticultural Science
1986 Volume.27 No. 4 p.314 ~ p.319
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The Use of Isozyme analysis to Determine the Purity of Cucumber (Cucumis sativus L.) F1 Hybrid Seeds
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Abstract
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Soluble proteins extracted from cotyledons of imbibed cucumber seeds of three commercial F₁ hybrid varieties(H.N. Semi-White; Black Pearl; Summer Fresh) and their six parental inbred lines were separated by starch gel electrophoresis and stained for six enzymes(Acid phosphatase; Malate dehydrogenase; Phosphoglucomutase; Phosphoglucose isomerase; Malic enzyme; 6-Phosphogluconate dehydrogenase) to investigate the possibility of utilizing isozyme variants to determine sib frequencies in an F₁ hybrid variety.
The results are summarized as follows:
1. Electrophoresis techniques necessary to separate isozymes of six enzymes presented in imbibed cucumber seeds have been established.
2. Diagnostic band variants named MDH-1 were found in the most anodal zone for Malate dehydrogenase from the parents of F₁ variety H.N. Semi-White.
3. The band pattern of MDH was stably detected during the germination of four daps and the activity of MDH was high enough for F₁ purity test at two days of seed imbibition.
4. The analysis of the F₂ and backcross progeny revealed that MDH-1 variants are controlled by co-dominant alleles(MDH-1^F, MDH-1^S) at one locus.
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